The use of hCG

hCG is administere primarily by intramuscular injection (IM), but also subcutaneously (SC).

The average (3ml 22-25G x ½-1 ½”) syringe was sufficient for IM injection. But an insulin syringe (½-1ml 28-30G x ½-1″) was recommended for SC injection.

The study found little difference in results between intramuscular and subcutaneous injections. The only difference is the speed of release at the injection site and the time required to reach the plasma peak. Intramuscular injection is 6 hours, subcutaneous injection is 16-20 hours.

If used to treat hypogonadism, short-term treatment is currently recommended for 6 weeks and long-term treatment for 1 year, depending on individual needs.

Short-term medication is usually given at 500-1000 units three times a week for three weeks. Then the dose was the same twice a week for 3 weeks.

Long-term injection of 4000 units, 3 times a week, for 6-9 months. This was then reduced to 2,000 units three times a week for three months.

hCG is sealed in a vial or ampoule as a freeze-dried powder (freeze-dried). The commonly used hCG specifications are 2000IU, 5000IU and 10000IU.

hCG freeze-dried powder should be stored at room temperature between 15 and 30 degrees Celsius. It should be reformulated with appropriate bacteriostatic water (or sterile water) before administration. The prepared solution needs to be refrigerated at a temperature of about 2-8 degrees Celsius.

The prepared hCG solution should be used within 30 days as far as possible.

When preparing hCG solutions, the concentration of hCG per ml of solution depends on the concentration of freeze-dried powder and the volume of thinner. For example, if 5000IUs is diluted with 5ml of solvent, the resulting solution contains 1000IUs per ml. Similarly, if 5000IU hCG is diluted with 10ml of solvent, the resulting solution contains 500IU/ml of hCG.

Bacteriostatic water should be used as a solvent when preparing a 30-day dose at a time.

Because sterile water diluted solution is more likely to breed bacteria than oil solution, always clean the top of the bottle with alcohol thoroughly when injecting the solution, and the needle used for injection must also be sterile.

Due to the fragility of protein hormones, if kept at room temperature after preparation, the molecules will denature and be destroyed, and hCG will become ineffective.

Violent shaking of the recombinant hCG will also destroy the protein molecules and should be avoided during preparation.